Species Reactivity : Human (Homo sapiens)
UniProt : Q53FZ2
Abbreviation : ACSM3
Alternative Names : SA; SAH; SA (rat hypertension-associated) homolog|SA hypertension-associated homolog
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 3.9 pg/mL
Intra-AssayCV : ?4.0%
Inter-AssayCV : ?7.2%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ACSM3 in samples. An antibody specific for ACSM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ACSM3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : By Southern analysis of somatic cell hybrids, Szpirer et al. (1993) independently assigned the Sa gene to rat chromosome 1 and showed that the human homolog, SAH, resides on chromosome 16. the deduced amino acid sequence from the isolated human SAH cDNA consisted of 578 amino acid residues and had slight homology to a bacterial enzyme, acetyl-CoA synthase. With the restriction enzyme PstI, they found a RFLP in the SAH gene and compared allele frequencies between hypertensive and control groups. The hypertensive group consisted of 89 persons, and the PstI rare allele (allele A2) frequency in this group was 0.270. The control group consisted of 81 healthy normotensive persons, among whom the A2 allele frequency was 0.09. The differences were significant at the P = 0.0001 level.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).