Species Reactivity : Human (Homo sapiens)
UniProt : Q86TH1
Abbreviation : ADAMTSL2
Alternative Names : FLJ45164; KIAA0605;
Application : ELISA
Range : 3.12-200 ng/mL
Sensitivity : 0.78 ng/mL
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.7%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ADAMTSL2 in samples. An antibody specific for ADAMTSL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTSL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ADAMTSL2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : ADAMTSL2 encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) and ADAMTS-like protein family. Members of the family share several distinct protein mod µLes, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene lacks the protease domain, and is therefore of a member of the the ADAMTS-like protein subfamily. It is a secreted glycoprotein that binds the cell surface and extracell µLar matrix; it also interacts with latent transforming growth factor beta binding protein 1. Mutations in this gene have been associated with geleophysic dysplasia.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).