Volume : 50 µg
Clone Number :
Aliases : E1A associated protein p300 antibody; E1A binding protein p300 antibody; E1A-associated protein p300 antibody; EP300 antibody; EP300 : E1A binding protein p300 antibody; EP300_HUMAN antibody; Histone acetyltransferase p300 antibody; KAT3B antibody; p300 HAT antibody; RSTS2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q09472
Immunogen : Recombinant Human Histone acetyltransferase p300 protein (2283-2414AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Functions as histone acetyltransferase and reg µLates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene reg µLation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stim µLates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 thro µgh acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and mod µLation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Reg µLates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-reg µLated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed : 10733570, PubMed : 11430825, PubMed : 11701890, PubMed : 12402037, PubMed : 12586840, PubMed : 12929931 PubMed : 14645221 PubMed : 15186775, PubMed : 15890677, PubMed : 16617102, PubMed : 16762839, PubMed : 18722353, PubMed : 18995842, PubMed : 23415232, PubMed : 23911289, PubMed : 23934153, PubMed : 8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed : 20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed : 24939902). Acetylates MEF2D.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : EP300
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Metabolism; Stem cells
