Species Reactivity : Human (Homo sapiens)
UniProt : Q6FIF0
Abbreviation : ZFAND6
Alternative Names : AWP1; ZA20D3; ZFAND5B; protein associated with PRK1|zinc finger; A20 domain containing 3
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.056 ng/mL
Intra-AssayCV : ?5.8%
Inter-AssayCV : ?10.9%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ZFAND6 in samples. An antibody specific for ZFAND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFAND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ZFAND6 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFAND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The deduced 208-amino acid AWP1 protein has a calc µLated molec µLar mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains : ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic retic µLum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).