Volume : 50 µg
Clone Number :
Aliases : bHLHe8 antibody; Circadian locomoter output cycles kaput protein antibody; Circadian locomoter output cycles protein kaput antibody; Circadian Locomotor Output Cycles Kaput antibody; Circadium Locomotor Output Cycles Kaput antibody; Class E basic helix-loop-helix protein 8 antibody; CLOCK antibody; Clock circadian reg µLator antibody; Clock homolog antibody; Clock protein antibody; CLOCK_HUMAN antibody; hCLOCK antibody; KIAA0334 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O15516
Immunogen : Recombinant Human Circadian locomoter output cycles protein kaput protein (577-846AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, reg µLates various physiological processes thro µgh the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots \'circa\' (about) and \'diem\' (day) and acts as an important reg µLator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovasc µLar, and renal function. Consists of two major components : the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for \'timegivers\'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks thro µgh neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molec µLar level by reg µLating gene expression to create a peak of protein expression once every 24 hours to control when a partic µLar physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1 ARNTL2/BMAL2, PER1 PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the µLtradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovasc µLar diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molec µLar circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5\'-CACGTG-3\') within their promoters. The core clock genes : PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively reg µLating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and nonhistone proteins, including its own partner ARNTL/BMAL1. Reg µLates the circadian expression of ICAM1 VCAM1 CCL2, THPO and MPL and also acts as an enhancer of the transactivation potential of NF-kappaB. Plays an important role in the homeostatic reg µLation of sleep. The CLOCK-ARNTL/BMAL1 heterodimer reg µLates the circadian expression of SERPINE1/PAI1 VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1 PPARGC1A, PPARGC1B, SIRT1 GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1 ATF4, MTA1 KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of m µLtiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, reg µLating the DNA accessibility of other transcription factors. The CLOCK-ARNTL2/BMAL2 heterodimer activates the transcription of SERPINE1/PAI1 and BHLHE40/DEC1.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CLOCK
Research Areas : Epigenetics and Nuclear Signaling; Neuroscience; Cancer; Cardiovasc µLar; Metabolism; Signal transduction
