Volume : 50 µg
Clone Number :
Aliases : 39 kDa protein kinase antibody; CAK antibody; CAK1 antibody; Cdk activating kinase antibody; CDK-activating kinase 1 antibody; CDK-activating kinase antibody; cdk7 antibody; CDK7_HUMAN antibody; CDKN7 antibody; Cell division protein kinase 7 antibody; Cyclin dependent kinase 7 antibody; cyclin-dependent kinase 7 (MO15 homolog; Xenopus laevis; cdk-activating kinase) antibody; Cyclin-dependent kinase 7 antibody; HCAK antibody; Homolog of Xenopus MO15 Cdk activating kinase antibody; Kinase subunit of CAK antibody; MO15 antibody; MO15; Xenopus; homolog of antibody; P39 Mo15 antibody; p39MO15 antibody; Serine threonine kinase Stk1 antibody; Serine/threonine protein kinase 1 antibody; Serine/threonine protein kinase MO15 antibody; STK1 antibody; TFIIH basal transcription factor complex kinase subunit antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P50613
Immunogen : Recombinant Human Cyclin-dependent kinase 7 protein (212-341AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression thro µgh the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1 POLR2A, p53/TP53, CDK1 CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1 CDK2, CDK4 and CDK6 by threonine phosphorylation, thus reg µLating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant thro µghout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cell µLar growth inhibition.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CDK7
Research Areas : Epigenetics and Nuclear Signaling; Cell biology
