Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q2KHW8
Abbreviation : GEMIN6
Alternative Names : FLJ23459; gemin 6
Application : ELISA
Range : 7.81-500 pg/mL
Sensitivity : 2.7 pg/mL
Intra-AssayCV : ?3.6%
Inter-AssayCV : ?6.9%
Recovery : 0.95
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate GEMIN6 in samples. An antibody specific for GEMIN6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGEMIN6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for GEMIN6 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GEMIN6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : GEMIN6 is part of a large macromolec µLar complex, localized to both the cytoplasm and the nucleus, that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN , GEMIN2 (SIP1), GEMIN3 (DDX20), GEMIN4, and GEMIN5. The deduced 167-amino acid protein has a predicted molec µLar mass of 18 kD and shares 86% sequence identity with the mouse homolog. GEMIN6 migrates as a 16-kD protein by Western blot analysis of affinity purified SMN macromolec µLar complexes. Immunofluorescence microscopy of HeLa cells showed GEMIN6 staining in both the cytoplasm and the nucleus with a high concentration in gems.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).