Species Reactivity : Deer
UniProt : P07353
Abbreviation : IFNG
Alternative Names : N/A
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?9.5%
Recovery : 0.94
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IFNG in samples. An antibody specific for IFNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IFNG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Interferon gamma (IFN-gama ) is a m µLtifunctional protein first observed as an antiviral activity in c µLtures of Sindbis virus-infected leukocytes stim µLated by PHA . Produced by T lymphocytes and natural killer (NK) cells, IFN-? is now known to be both an inhibitor of viral replication and a reg µLator of numerous immunological functions.IFN-? influences the class of antibody produced by B cells, upreg µLates class I and II MHC complex antigens and increases. the efficiency of macrophage-mediated killing of intracell µLar parasites. Most of the activities attributed to IFN-? are believed to be mediated by IFN-?-induced proteins. The appearance of such proteins is a consequence of IFN-? binding to a specific receptor that is distinct from the receptor for IFN-?and?.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).