Species Reactivity : Fish
UniProt : N/A
Abbreviation : CH
Alternative Names : N/A
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.2%
Inter-AssayCV : ?9.2%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CH in samples. An antibody specific for CH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CH is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Cholesterol is a lipid found in the cell membranes of all animal tissues, and is transported in the blood plasma of all animals. Cholesterol is also a sterol. Because cholesterol is syntheVolumed by all eukaryotes, trace amounts of cholesterol are also found in membranes of plants and fungi. The name originates from the Greek chole- (bile) and stereos (solid), and the chemical suffix -ol for an alcohol, as researchers first identified cholesterol in solid form in gallstones by François Po µLletier de la Salle in 1769.Most of the cholesterol in the body is syntheVolumed by the body and some has dietary origin. Cholesterol is more abundant in tissues which either syntheVolume more or have more abundant densely-packed membranes, for example, the liver, spinal cord and brain. It plays a central role in many biochemical processes, such as the composition of cell membranes and the synthesis of steroid hormones.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).