Species Reactivity : Goat (Capra hircus; Caprine)
UniProt : N/A
Abbreviation : E2
Alternative Names : N/A
Application : ELISA
Range : 40-1400 pg/mL
Sensitivity : 18 pg/mL
Intra-AssayCV : ?3.8%
Inter-AssayCV : ?7.6%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Competitive ELISA
Analysis Method?? : Quantitive
Test principle : This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conj µgated E2 and incubated. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview : Estradiol is an estrogenic hormone, C18H24O2, produced by the ovaries and used in treating estrogen deficiency. Estradiol is essential for maintaining normal female functions and is normally produced by the ovaries. After menopause, the ovaries decrease their production of this hormone. Estradiol is a sex hormone. Estradiol is the predominant sex hormone present in females; however, it is present in males, albeit at lower levels, as well. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.Estradiol is produced not only in the gonads : In both sexes, precursor hormones, to be specific testosterone, are converted by aromatization to estradiol. In partic µLar, fat cells are active to convert precursors to estradiol, and will continue to do so even after menopause.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).