Species Reactivity : Human (Homo sapiens)
UniProt : O15392
Abbreviation : BIRC5
Alternative Names : API4; EPR-1; apoptosis inhibitor 4|bac µLoviral IAP repeat-containing protein 5|survivin variant 3 alpha
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 12.4 pg/mL
Intra-AssayCV : ?3.7%
Inter-AssayCV : ?7.3%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate BIRC5 in samples. An antibody specific for BIRC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBIRC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for BIRC5 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIRC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Survivin is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative reg µLatory proteins that prevent apoptotic cell death. IAP family members usually contain m µLtiple bac µLovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain.The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in ad µLt tissues. Antisense transcripts are involved in the reg µLation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the f µLl-length natures of only three of them have been determined.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).