Species Reactivity : Human (Homo sapiens)
UniProt : Q13936
Abbreviation : CACNA1C
Alternative Names : CACH2; CACN2; CACNL1A1; CCHL1A1; CaV1.2; MGC120730; TS; DHPR; alpha-1 subunit|OTTHUMP00000196730|OTTHUMP00000196731|OTTHUMP00000196732|OTTHUMP00000196733|OTTHUMP00000196734|OTTHUMP00000196735|OTTHUM
Application : ELISA
Range : 0.78-50 ng/mL
Sensitivity : 0.4 ng/mL
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?7.9%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CACNA1C in samples. An antibody specific for CACNA1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCACNA1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CACNA1C is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CACNA1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Activation of voltage-sensitive calcium channels by membrane depolarization triggers key cell µLar responses such as contraction, secretion, excitation, and electrical signaling. The L-type currents produced by voltage-sensitive calcium channels are blocked by 14-dihydropyridine (DHP) derivatives; thus, the channels responsible for these currents are referred to as DHP-sensitive. The skeletal muscle DHP-sensitive calcium channel is a complex of 5 subunits : alpha-1 alpha-2, beta, gamma, and delta. The DHP-sensitive calcium channels from cardiac muscle and the brain have pharmacologic and electrophysiologic properties that differ from those of the skeletal muscle channel. Powers et al. (1991) isolated a clone for the human CCHL1A1 gene and partially sequenced it.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).