Species Reactivity : Human (Homo sapiens)
UniProt : P06753
Abbreviation : TPM3
Alternative Names : RP11-205M9.1; FLJ41118; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; TRK; hscp30; cytoskeletal tropomyosin TM30|heat-stable cytoskeletal protein 30 kDa|tropomyosin
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.113 ng/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?7.3%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TPM3 in samples. An antibody specific for TPM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TPM3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Tropomyosin alpha-3 chain is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and reg µLate access of other actin-binding proteins. In muscle cells, they reg µLate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene res µLt in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. M µLtiple transcript variants encoding different isoforms have been found for this gene.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).