EasyStep Rat IGFBP7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit - 48 Tests

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP7, and the Rat IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat IGFBP7. After TMB substrate solution is added, only those wells that contain Rat IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.